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This phase begins from the moment in which the plants, duly conditioned, are placed in the mesh house. b. Irrigation: If the conditions are normal and there is no physiological wilt, the second maintenance irrigation is done 10 or 12 days after the transplant. This second irrigation is accompanied by a mixture of fertilizer rich in phosphorus to continue stimulating the development of the roots. Depending on the micro-climatic conditions in the mesh house and the state of turgor of the seedlings, one or two waterings can be scheduled daily. When this irrigation system is used, rigorous inspections must be carried out to detect any phytopathological problem. d. Fertilization: Every eight days the fertilization is done with a fertilizer rich in phosphorus, alternating it with a complete fertilizer that contains the major and minor elements. If there are specific symptoms of the deficiency of an element, foliar fertilization can be done with simple or complete fertilizers. F. Transplant to the final site: Between 2 and 3 months of staying in the mesh house, the plants are ready to be taken to the final site. During this period it is important to pay attention to nutritional deficiencies or problems of pests and diseases. In order to avoid contamination by fungal diseases, the seedlings are immersed for five minutes in a container containing a solution prepared with fungicide. A substrate is used for the transplant, which is sterilized. I always recommend using an express pot, since in addition to reducing sterilization times it is much more effective, especially with whole grain substrates. Sterilization time from one and a half hours -Olla express: It is the most effective and most widespread method. There are many express pots, but the operation will be the same for all. It will be necessary to put something in the bottom of the pot so that the glasses or jars do not receive too much direct heat and are exploded at the first exchange. It will range from a metal drainer, to a dish towel that burns the rag inside the pot. Consist in putting the glasses, adding 3cm of water more or less. It is quite subjective, since it depends on the size of the pot, glasses, etc. Although the water mediates the glasses nothing will happen, but more I think it could be risky. At this time we will reduce the heat and let sterilize from 1 hour to an hour and a half. There is a very visual way of knowing if the pressure inside the pot is optimal, and this is to throw a drop of water on the surface of the pot when you are already sterilizing and see what happens. If the drop of water begins to bubble and evaporates quickly, it will be doing well. At all times a strict phytosanitary control must be carried out using antibiotics, fungicides and insecticides of universal use. It is convenient to add fertilizers, either through the substrate or through the irrigation system; using proportions rich in phosphorus and potassium that will favor root development and rustification of plants. If the root system was differentiated in vitro plants can not be transplanted directly to greenhouse conditions without a gradual adaptation to soil conditions. This period of adaptation has been called hardening period. With woody, annual and succulent species, plants obtained in vitro should be carefully washed to remove all agar residues, which may be a source of contamination. During this hardening phase the plants are preferably irrigated with 50% diluted culture medium and this irrigation formula is subsequently replaced by less complex nutritive solutions. When the boxes arrived with the respective bottles, they were quickly removed from the boxes and placed separately in a cool place with adequate lighting. This is an opportunity to pre-select, eliminating bottles that are contaminated, broken or mistreated. Under these conditions a high mortality rate was observed. When this happens, it is recommended to store the jars in a cool place with adequate light, for 1 or 2 days. During this period, the plastic tape that surrounds the lid of the jars is removed to acclimate the seedling to the conditions of the place. In all cases, the substrate was steam sterilized to minimize the risk of the presence of fungi and soil nematodes. If you do not have the "sterilization" equipment, you can deposit the sand in a metal container containing water and then heat it. Regarding the soil, you can spread a thin layer of soil in a plastic, cover it with another black plastic in an airtight way and leave it a week exposed to the sun. In general, the formation of adventitious buds can lead to very high rates of multiplication, higher than those obtained from axillary branches. The different plants may respond in a different way to the various cytokinins and auxins in part due to their natural hormonal control. An appropriate sequence of hormones is of particular importance in that a hormone may have an inducing effect but must be absent or in a reduced amount for the organ to grow. Some callus developments are strongly lignified and hard on texture, which can not be easily separated into small fragments. On the contrary, fragile calluses are easily separated and are called friable, fragile cultures. The calluses can be yellowish, white, green, or colored with anthocyanins. The pigmentation will be throughout the callus, or in some regions without pigmentation. Its anatomy is of considerable variation throughout the cellular differentiation. The first scholars assumed that the cultivation of calluses derived from chlorophyll-containing organs may be autotrophic in their nutrition. Calli with chlorophyll, in any way, are dependent on exogenous sugar to continue growing, even with adequate intensity of light. After the callus has grown associated with the original tissue for a while, it becomes necessary to pass it to a fresh medium. The growth in the same medium for an extended period will cause the depletion of nutrients and a gradual desiccation of the agar by the loss of water. Metabolites secreted by calluses can accumulate in toxic levels in the environment. The transfer of the callus fragment must be sufficient to ensure the new growth in the fresh medium. If the transferred inoculum is very small, it will exhibit a very low growth rate, or it will not grow. Some authors recommend that the inoculum should be 5-10 mm in diameter and weigh 20-100 mg. The successive peals are made every 28 days in culture tubes that have 30 cm3 of medium. The time between rings is variable and depends on the growth rate of the callus. The parenchyma is inefficient, performs little photosynthesis. Stomata do not close at 100% and leak water. It is stated that the tissues of tropical plants can be expected to grow better at temperatures higher than those appropriate for tissue culture of temperate zones. In general in tissue culture, temperatures ranging between 24ºC and 28ºC are used. It is also important to remember the relationship of temperature with events such as morphogenesis and the concentrations and action of growth regulators. The wavelength and light intensity must be taken into account when tissue culture is performed. The alternation of light and darkness should be studied in each case, although in tissue culture, a photoperiod requirement similar to extra-vitro grown plants can be presented. It has been shown in some plants that the length of the day influences the endogenous levels of auxins and cytokinins. Analogously, the number of hours of light received by the cultivated in vitro can affect its development. In general, the best in vivo photoperiod will also be the best in vitro photoperiod. In general, white light and a photoperiod of 16 hours of light and 8 hours of darkness are used. The incubation temperature for the propagation of most families fluctuates between 24 and 28 ° C. The temperature regimes have varied in the day and in the night, and it has been found that only in a residue number of species such variation is advantageous. Recent research has proven that light is a fundamental factor in morphogenesis. In this regard, it has been observed, in the case of Pinus radiata, that light interacts with a cytokinin during the differentiation of adventitious shoots and that morphogenesis does not occur when one of these two components is missing. The role of light in differentiation involves several components such as intensity, photoperiod and quality: although the morphogenetic importance of these components is recognized, studies carried out with the purpose of analyzing them are scarce. inert substances are added only for the position and better development of the explant. phytagel is added, or else agar to solidify it. formerly liquid media were used as extracts or organic complexes, such as coconut water, yeast extract, etc. The consistency of the culture medium can be used as semi-solid or liquid. The most used gelling agent in the in vitro culture is the agar extracted from various marine algae. The different qualities existing in the market modify the morphogenic expression because they can contain inhibitory substances or growth promoters. In case of selecting liquid culture media, the observed morphogenic response varies considerably. Liquid culture is essential when seeking to promote indirect morphogenesis through cell suspensions. For this it is necessary to cultivate the callus in liquid medium with agitation to allow the cells to disintegrate and can differentiate roots, buds or somatic embryos in isolation. The selection of the liquid or solid culture medium also depends on the species used. In Cymbidium spp, for example, it has been observed that the use of liquid culture medium promotes a greater differentiation of protocorms with respect to the same gelled medium. In turn, this process can be maintained during several subcultures while in gelled culture medium it was observed that the protocorms tend to form stems. If this equipment is not available, it can be replaced with rooms previously sterilized with ultraviolet light. The work table and the walls of the cabinet must be previously disinfected with 70% ethanol. In the same way, all containers that enter the sterile air area must be disinfected externally. Operators are frequently an important primary source of contamination, because it is recommended that, before starting work, wash their hands and forearms with plenty of water and soap and de-infect with 70% ethanol. The use of dust covers, gloves and protective masks of the mouth and nose, as well as the protective caps of the hair, help to reduce significantly the levels of contamination. Work instruments must be sterilized before use. Many of these instruments can be placed in 95% ethanol and, before being used, should be flamed carefully in the flame of a lighter. It is also necessary to flap the mouth of the containers containing the culture media before and after culturing the explant. Incubate the crops in chambers or rooms of culture, closed, free of drafts and well sanitized. Where possible, the movement of people should be restricted, and containers with contaminated crops should be quickly eliminated. of this sector. It is convenient that before washing, these crops are sterilized. Sodium hypochlorite solution from 5 to 20%, between 5 and 30 min. Rinse with plenty of sterile H2O. In the case of HgCl2, the material must be rinsed several times as it is difficult to remove. The selection and concentration of the disinfectant and the disinfection time are determined to a large extent by the characteristics of the explant; in practice they are mainly established by trial and error. After disinfecting the explant, it is sown in the culture medium with the help of the forceps, taking care not to introduce the hand to the bottle and let the yolk remain upright, after that the bottle is closed and sealed. The explant must respond efficiently under in vitro conditions. It is important to consider the fact that the isolation of a tissue or organ from the rest of the plant causes a state of stress that alters its cellular metabolism and importantly its hormonal balance. A good explant is one whose cells survive in a high portion, to the decomposition indicated above, and which then efficiently responds to in vitro conditions. However, the larger the size of the explant, the greater the chances that the cultures will be contaminated with microorganisms. It is also necessary to bear in mind that there is a minimum size of the explant, which depends on the species and plant material, below which it is not easy to achieve the establishment of crops. Very small explants often require the use of more complex media or so-called conditioned media. If there are differences, they can be explained generally by the fact that on the sheet, there are tissues of different ages. On the other hand, great differences have also been found, according to the zones, in the regeneration capacity of some organs. The type of explant to be used in the micropropagation processes depends on the species with which one is working and the objectives that are pursued. Explants such as apical meristems and axillary buds are genetically very stable, this type of explants serves to reproduce multiple clones of a form or variety with special characteristics that you want to keep in the crop. With the exception of the ovules and pollen, the explants are constituted by tissues and somatic cells. The name "explant" is a Castilianized version of the English word "explant"; specially coined to identify plant tissues grown in vitro and without other meaning. As a general rule, it can be said that the younger and undifferentiated the explant to be cultivated, the better its in vitro response. That is why the apical and axillary meristems are widely used in numerous species. In the case of the micropropagation of woody plants, the age of the explant is a critical factor. If the tissues are young, micropropagation is more likely to be successful than with mature tissues. This fact generates the need to perform rejuvenation treatments of explants-giving plants. Light, temperature and relative humidity are the main environmental factors that affect crops. The behavior of many crops depends on the quality, intensity and photoperiod of the light they receive, given that several enzymes involved in the development and secondary metabolism are influenced by light. Most of the crops develop at a light intensity between 5 to 25 W m 2. While the quality of the light can determine different morphogenic responses, in general white light is used, poor in long wavelengths. The photoperiod usually used is 16 hours of light and 8 hours of darkness, although some crops require darkness. The temperature of the cultivation chambers is generally regulated between 22 and 28 ° C, thus allowing the development of both temperate and tropical plants. The speed of synthesis and degradation of different compounds and therefore the level of production and accumulation of metabolites is also influenced by temperature. As for the relative humidity, it remains at around 70% under the conditions in which crops are usually carried out, although it varies with the temperature of the chamber and the type and closure of the containers. The percentage of relative humidity is not reported in most works on in vitro cultivation of vegetables. Juvenile tissues have a greater morphogenetic capacity, which manifests itself in response to growth, proliferation and rooting, unlike a mature tissue which is more difficult to de-differentiate and induce the production of roots and shoots. Much of the success of tissue culture is in the age of the explant that will be used for the works. The age of the plant and the degree of tissue differentiation are often interrelated, and can produce interactive effects when it is grown in vitro. Likewise, the production of adventitious buds in leaf explants of Cucumis melo, showed higher production in the explants that had a size between 0.3 and 0.5mm which had 14 days of planting. This property did not have those explants that were more than 21 days old. Physiological or ontogenetic age: O phase of growth. Includes the concepts of youth and adult. The degree of differentiation: With this concept, the younger parts of the plant are undifferentiated meristematic cells. Theoretically it is said that some plants have meristematic cells always in a youthful state and that they could live forever. Cultivation period: It is the time since the culture was just installed. In addition, you should look for plants that are in a vegetative state. It is very common to use tissue culture to obtain plants free of viruses. The ideal explant for this is the consistent meristem of the dome and a pair of leaf primordia. In order to avoid and minimize the contamination of crops with microorganisms, it is necessary to: 1. Know the plant material with which you work and the possible specific contaminants. Carry out an adequate preparation of the explants plant, cultivating it preferably in greenhouses treated with chemical products that eliminate pathogens and possible endophytic microorganisms. Proceed to the superficial disinfection of the explants by using chemical compounds in order to eliminate microorganisms with the least possible damage to the explant. Some procedures are based on the use of only ethanol or sodium hypochlorite. Finally, it is necessary to eliminate the remains of these products by several washes with sterile distilled water. The donor plant should be chosen based on a positive mass selection for the desirable agronomic characteristics. Once the individuals have been selected, it is necessary to define the type of explanation to be established under in vitro conditions. In general, young or rejuvenated organs are those that have a better response in the establishment than those obtained from adult materials. The use of explants that are exposed to low levels of pathogens can solve the problem of fungal and bacterial contamination during the establishment of the in vitro culture. It is recommended to collect primary explants in the field during the spring and summer season, when there is an active sprouting of the buds, since the use of dormant buds causes serious problems of contamination. In order to achieve optimal quality explants, it is convenient to grow the donor plants for a minimum time under greenhouse conditions. In this way, it is possible to directly influence the health status and the quality of explants by controlling the light intensity, temperature and regulators of growth. By controlling these factors it is also possible to obtain more homogeneous donor plants and explants throughout the year. In addition, pretreatments with growth regulators can be applied to the donor plants, as well as to the explants themselves. In woody species, the immersion of the primary explants in solutions with cytokinins is usually used as a pretreatment in order to induce the sprouting of buds. Once the mother plant has been chosen, the fragments from which the explants will be obtained will be extracted. Before extracting the explants, disinfection of the mother plant fragments will be done to eliminate external contaminants. The most common contaminants are fungi and bacteria that live naturally in the environment. Once the plant material has been disinfected, it must be maintained in aseptic conditions. In order to obtain the conditions of asepsis, we will work in laminar flow cabinets to extract the explants from the plant material. After surface disinfection, the seeds or buds depending on the selected material are placed in a sterile culture medium. In a period of one week or fifteen days, the process of germination or regeneration of new plant tissues begins, initiating the in vitro culture cycle. Knowing the expenses that are generated in this phase can save resources if the plants are extracted with the appropriate size and acclimate better when they are maintained 45 days under the climatic conditions of the territory. The above agrees with what Suárez referred to in the organization of production in Cuban biofactories in acclimatization areas, where values between 95-98% of survival are admissible for this phase. These results coincide with those of Pérez et al. that recommend that the management of plants in this phase should be gradual and without creating traumas, if you want to obtain plants of sufficient quality and homogeneity to be taken to the field. In this period, the greatest amount of losses occurs and, depending on the crop, after 30 days, survival rates of over 90% can be achieved. To root the explants, we use mainly individual seedlings with an approximate size of 2 centimeters. The shoots obtained during the multiplication phase are transferred to a medium free of growth regulators or that only contains hormones of the auxin type. Some plant species do not need to go through this stage and emit their roots in the same culture medium where they develop new buds, therefore the multiplication and rooting process take place simultaneously. The main objective of this stage is to maintain and increase the number of outbreaks for the new successive multiplication cycles and to allocate part of them to the next stage of production. To obtain these explants, it is advisable to maintain the mother plants, that is, the plant that donates the yolks, for a period of time that can range from a few weeks or several months in a greenhouse under controlled conditions. In this environment, the plant is cultivated under optimal sanitary conditions and with adequate nutrition and irrigation control to allow vigorous and disease-free growth. It is the aseptic establishment of plant tissues, with a specific purpose: • Cleaning of viruses and other pathogens. The formulation of the medium changes depending on whether you want to obtain a differentiated tissue, grow buds and roots, or obtain somatic embryos to produce artificial seeds. The success in the propagation of a plant will depend on the possibility of expression of the total cellular potential, that is to say, that some cells recover their meristematic condition. To this end, the differentiation must be induced first and then the cellular redifferentiation. A process of this kind happens during the formation of the adventitious roots in the rooting of cuttings, the formation of adventitious buds, or when looking for the propagation of begonias, African violet opeperonias through portions of leaves. One of the most important factors to take into account to achieve the desired morphogenetic response is the composition of the culture medium. The establishment consists of the disinfection of the explants and their subsequent adaptation to the artificial environment in order to induce callus, sprout, root or somatic embryo, as desired. It is necessary to disinfect the chosen material superficially to avoid microorganisms growing in the culture medium, mainly bacteria and fungi, which will compete advantageously with the explanto. This announcement, awaited by the scientific community in recent years, offers an opportunity to make a series of reflections that are of particular importance for the current society in which we live. Gamba Ayala said that the stem cells are originally the cells that were all of us during the embryonic stage. They are cells that have the ability to differentiate into various types of adult cells. However, in order to even think about this possibility, the first thing we need to have in our hands are the pluripotent cells. Gurdon and Yamanaka showed that by manipulating a few genes it is possible to take a specialized adult cell and induce it to become a pluripotent cell. These cells were called inducible pluripotent cells. Thus, they took fibroblasts from an adult mouse and converted them into iPS. In this way, embryos are no longer required and the rejection problem is eliminated, because the cells come from the same patient. These cells are present in the embryonic stage, and have the ability to differentiate into various types of adult cells. In other words, the nucleus of the gut cell still "remembered" how to be pluripotent, just put it in the right medium. On the other hand, in 2006, Shinya Yamanaka and her group demonstrated that by manipulating a few genes it is possible to take a specialized adult cell and induce it to become a pluripotent cell. Therefore, from these two findings, the possibility opened up to the future of taking specialized cells from an adult, and later deriving them to form the cells that are needed to regenerate other tissues. Generally, a vapor pressure of one atmosphere above normal pressure is used, which corresponds to a temperature of 121 ° C. The penetration power of steam at this temperature is very high, enough to destroy in 15 to 20 minutes, depending on the volume of the material to be sterilized, spores that would survive boiling for hours. There are several types of autoclaves, Chamberlain being the most commonly used. The boiler has a removable and perforated double bottom on which the material to be sterilized is placed. The upper part closes with a bronze lid that fits perfectly through a silicone rubber gasket and a series of bronze screws. When the autoclave is used with saturated steam at higher than normal pressure, the following steps should be followed: 1. Place water in the boiler, trying to ensure that its level does not reach the objects that will be placed inside it. Only the steam that is the sterilizing agent must reach the material to be sterilized. Load the material to sterilize properly conditioned. Close the autoclave by adjusting the screws of its lid in a crossed position, in order to ensure a close and airtight closing. In this way the useful life of the silicone gasket is prolonged, the thickness of which must be homogeneous throughout the entire travel. Open the steam outlet and turn on the heat source. Ensure the correct condition of the safety valve. The steam outlet must be open to dislodge the interior air, until a continuous and abundant jet of steam comes out. Temperature working, 121 ° C will be reached at the pressure of one atmosphere above normal only if steam exists in the autoclave in equilibrium with water, so it is necessary to ensure the elimination of air inside the boiler. If proper air removal is not achieved, the presence of air mixed with water vapor will result in a temperature below 121 ° C. From what has been said it can be deduced that the pressure gauge will be able to mark an atmosphere of overpressure, but if a correct air removal has not been carried out, the temperature will be lower than expected. Purge the appliance: the steam outlet cock is closed and opened three times to eliminate air traces. Heat until the pressure gauge indicates the desired pressure. Regulate the heat source to maintain constant pressure. From that moment start to count the sterilization time. Only then can the steam outlet key be opened and then the autoclave lid to remove the material. Flowing steam or steam at normal pressure The autoclave can be used using saturated steam at 100 C. In this case the open steam exit cock is used. When a continuous and abundant jet of steam comes out, the device is purged and the key is left open. From that moment, the exposure time is counted. This process known as tyndalization uses the intermittent action of steam or fractional sterilization. It is used for the sterilization of materials that do not withstand temperatures above 100 ° C. The process consists of a heating at 100 ° C for 30 minutes in which the bacteria die in a vegetative state. Then it is left at room temperature overnight and the operation is repeated, it is left again at room temperature and a third and final heating is carried out. The method is based on the fact that during the first heating the vegetative forms are destroyed and the sensitization of the sporulated forms is stimulated by the action of the temperature and the liquid medium. In subsequent heating, the sporulated forms, now sensitized, will be destroyed. In many respects an autoclave looks like a homemade pressure cooker. In fact, in some small demicrobiology laboratories these pots are used instead of autoclaves. Both are metal containers with walls strong enough to withstand the vapor pressure. Management of Transplanted Material 126.96.36.199. Transplant to Substrate 188.8.131.52. Preadaptation and Transplant 184.108.40.206. Growth of Adventitious Buds 220.127.116.11. Physiological Changes in the Explante 18.104.22.168. Conditions of Incubation 22.214.171.124. Dissection of the Explante 126.96.36.199. Disinfection of Explant 2.3.4. Position of the explant in the plant 188.8.131.52. Plant Growth Conditions 184.108.40.206. Selection of Mother Plants 220.127.116.11. Aseptic Establishment 2.3.1. Stages of tissue culture 2.3. Sterilization of Culture Media 2.2.6. Sterilization with Humid Heat 2.2.4. Preparation of Culture Media 2.1.5. Inert and gelling materials 18.104.22.168. Components of the Culture Medium 22.214.171.124. Types of Culture Media 126.96.36.199. Definition of Culture Media 2.1.3. Generalities of the Culture Media 188.8.131.52. Laboratory equipment 184.108.40.206.
I propose to Brother IB that when I can I can post on this topic. It is a matter of making numbers, if you think you can buy cheaper is worth selling, although this is impossible to know. But this is impossible to know. The proposal that I make to you is very simple: 1º.- do nothing. At a minimum it has a high psychological cost. That is to say, with what we obtain that year, we pay the insurance and if the dreaded fall occurs, we earn a very important money. Other options such as selling futures or other products I see much more risky, in that situation I prefer to limit the expense to a certain amount, lest I make a mistake and that continues to rise. We all have portfolios with the majority of companies "decent". Surely the beta on the index is low and can go cheaper. The price depends above all on how demanding you are with the insurance. The futures discount the dividend as "already given". So if you sell one to protect you lose the dividend of the period as well. I have voted and you have not registered my vote. We will see what we do if we enter a bullish movement of years or on the contrary the black swan goes out and returns us again to hell. More and more voices are warning that an important correction is going to arrive there, I also believe it in view of the graphics, they are already beyond Pluto. The content on this site is provided without any warranty, expressed or implied. No material here is investment advice nor is it a recommendation to buy or sell any financial instruments, including but not limited to stocks, options, bonds or futures. Actions you undertake as a consequence of any analysis, opinion or advertisement on this site are your sole responsibility. Tweet Follow this blog Theme images: enot-poloskun.
I have not posted anything for a while, and that's fine. Either way I felt I could not do this without a closure. Yes without a balance; that is precisely what I will omit now. A year ago this blog is a powerful two-part wall through which a flow of tikitikis is channeled into two or three different keyboards. I think it's nothing more than that. The reason why tikitikis become, sometimes organized and sometimes not so much, is open to a study alien to the very purpose of the blog and therefore does not correspond to explain it here either. This is a very valuable medium and I appreciate it; that's why I needed a rusty gold brooch to make it look like at the end of the year gala dinners. Without more: happy 2013, and come back soon! At the bottom of the neighborhood are the only willows that I tolerate. In the autumn naps I throw myself around without looking at them; by chance scattered near the river, you can always feel its breeze intuitively even if you do not see it. And if one approaches, he begins to feel the stench of rust from the shipyard thirty years ago abandoned. I say it by comparing it with my grandmother's tea, which I hear she is stirring now; and although I would smell it if I went up the stairs, I do not see it. The tea being stirred at the bottom of my house or the willow being stirred by the wind at the bottom of my neighborhood; the change of scales can happen at any scale. Think of an iceberg being split in half by some battleship, deep in the world; Think of the last sandy stone of Pluto or that louse that hangs from the right ear of your poodle dog. The last will be the first, they tell me and I do not understand. But as Siddhartha discourages the use of words, I also try to understand everything by facts. But its beauty makes us want to be there; If the willows in the back of the neighborhood are imagined by me, it is because they are calling me to visit them. I pick up a bag with badly sewn buttons from so much use. Feeling blue is a very special state. Although the study of color binds this color to the mind, the fact is that on many occasions blue inspires a state that transcends any mental border, taking us by the hand towards a versatile expansion that reminds us of metacorporality. Today: a musical recommendation. First step to describe your ideal relative situation, which does not go wrong reproducing the subject-recommendation at some wrong time. The morning in Corrientes is characterized by being as indulgently torrid as all the rest of the day, since the sun does not discriminate: it hates us all equally. And the great detriment that Corrientes has at a tourist level is not having one of those mornings in the whole summer season. What does not mean that it has its charm, but it is a misunderstood charm. Love him or hate him, it is inexorably cruel and while it brings us concessions and joys, it prevents us from doing other things. What Argentine does not want to get up at 7 in the morning with a bitter mate, and not chivar as miserable in order to satisfy this desire. The thing is that you should always look for ways to make everything lighter. But people do not notice and complain. What did not always exist, and what we do not know if it will be forgotten in future generations, is Thelonious Monk. Which is one of the antidotes for an unpleasant morning, but the world tends to forget antidotes. Incredibly, because it does not happen with all the themes, it has the same effect in the summer of the Litoral. There is no other way to achieve eternity than to delve into the moment, or another way to reach universality than through one's own circumstance: today and here. The task of the writer would be to glimpse the eternal values that are involved in the drama ¹ of his time and place. We made haikus as we climbed up the scrub-covered slopes. An authentic haiku has to be as simple as bread and, nevertheless, make you see real things. You clearly see the wet traces as a vision in your mind, and in those few words you also see all the rain that has been falling that day and you almost smell the wet needles. A good neuquino will know how long the desert is, the entrance road to a town called El Chocón. The only thing I can boast, and this just enlarging in front of a hypothetical reader who does not live in Patagonia, is to have known that blue lake that in memory seems to me one of the most beautiful things I saw. Now that I mention the way back, I do not remember how we left town. That day we arrived with very good humor. Diego, influenced by Into the Wild, was probably happy to bathe in the bluish lake and urged the other two to do the same; It was the most refreshing bathroom of my life. Sometimes, especially on cold days, I feel guilty for dirtying the lake with hair conditioner waste. Yes, it was downhill, and it did not help against the scorching sun. Our optimism was excessive, because they took us there after a wait of almost two days in a desolate wasteland, where there was only one service station, a hotel and an old pedophile who pretended to be a Mapuche cacique. But one stop, between magical and obligatory, was this small town of Neuquén. And while we were walking we could see, and this I did not say until now, the blue lake in the background. Surrounded by modest mountain ranges that had nothing to attract a noisy tourist, but instead, because of its simplicity, seduced us. Or perhaps only me, who took advantage of having learned the rhetorical figure of the plural also modest. I think I owe most of this unexpected melanin to that long walk that did nothing but placate our good humor. Our mood was declining, I say, progressively. It may be, but I can not be sure of having suffered from that bath in the blue lake. I did not realize it there, but putting things in perspective: my life has not been horrible again since that time, if it ever was. I do not remember a single moment in which I preferred death. The story of this entry ends when our relief begins; Dry pine trees began to grow on the route, and we saw a little farm with animals up close. I had time to digest your last epistle once read traveling all the way; I slept three hours in which I probably dreamed of you telling me everything, but I do not remember. When I woke up, I was in Resistance; everything was chillingly familiar. Making a rereading, which did not crave to be very different from the first ten hours before, I scribbled this while the warm collective allowed me in its inertia. It started when I was writing the footer, which is rather macabre and does not pass on complementary information. I feel heretical heir of these supermen who far from the apprehension that these extreme situations cause, have managed to overcome for their own calm: mental and spirit. Something inside tells me that I should smile at your departure; I obey without guilt. I do not intend to expand on this better. I do not know if it is a religion in a sadly dogmatic sense that we know; already last year I read that Borges cited a text in which he said that one could be a Hindu or a Buddhist being both a Christian or a Muslim. It is clear that it is not an inherent characteristic of the West to be so dichotomous, but it is instead a general malaise. Hinduism, according to what little I know, is not like that. Some time ago I was afraid to address the readings about this way of seeing the world, simply because I did not know where to start. Two weeks ago I do know, and now I am reading it systematically. The kick-off was The Dharma Beggars, a book full of very interesting passages that I was more or less adapting to my own needs, and I will approach a rereading at a time, to see if my needs happily changed. All this for what it's worth, I do not know. I also do not aspire to Buddhahood or nirvana, because I think that I am very contaminated, if I am not entirely made up of, the unhealthy essence of the West. The roast: the sacred cow of India. Another twist of the belief, poorly cultivated, that I hope to know a little more deeply. On this cloudy and warm Saturday I communicate: I am lost. I'm also lost inside the cat. The cat that has me as a slave cleaning his shit and caressing his ears, and me that he left me out of pure love. Like the time I went looking for him after a month without having him, and the cat bit me because he did not recognize me. Now that we are like father and son a little, a bit like roommates, and we already know all our tricks, we get along. The incongruous relationship is based on the fact that I am still in charge of buying food, which is a monumental effort especially when it ends on non-working days read cloudy Saturdays. I'm also lost inside the blog. And as I said in some previous post I do not like the things that I can not semantically apprehend completely; It's hard for me to start a novel if I did not read a review of how it ends. I do not like to be adrift, and with this blog I am not adrift but paddling without knowing where. I would like to be assigned a job, or a noble cause, but there is nothing. The blog is dead by definition. I'm paddling in a coffin, and meanwhile, collecting water lilies. I want to understand things through action. "Well, they have not told me anything, or maybe they can not tell me something useful, here is life, an experiment, most of which has not yet been done by me, but I do not benefit at all from what others have done, if I have some experience that I consider valuable, I am sure that my guides did not say a word about it. " it is not necessary to convince anyone of one's erudition, still less if one does not possess such erudition. The other day I told someone that you were always a spiritual company and now I am not less, except for the fact that I can no longer talk to you. So, as it is done with the gods, I have to make up opinions instead of consulting them. One of them is this: you do not want me to be wrong. And I get distracted by reading books, some of which remind me of you, and some of which are even marked with your own calligraphy. In short, you are only a divine cloud right now, nor are you less than that. Your hand with nails painted blue because that is the color that your mother detests. I never was, and this I already told you, in a position to understand you. Despite how well you say he did it. Because he talks about you, and you love him, because we love you. He thinks that I was never religious until today, day in which you seem to form in a way that resembles an unconscious tissue, several phenomena of my life. As if everything were indeed moved by your hand. In that case, you would be one of the vertices of my pantheism. We're going to miss you a lot, but I think less. For Europeans and Americans, there is an order - a single order - possible: the one that before took the name of Rome and that now is the culture of the West. Being a Nazi is, in the long run, a mental and moral impossibility. Nazism suffers from unreality, like the infernos of Erigena. It is uninhabitable; men can only die for him, lie for him, kill and blood for him. No one, in the central solitude of his self, can hope for success. I risk this conjecture: Hitler wants to be defeated. Independence sounds like a street in the center of Córdoba, which divides the streets into two different names; nothing more than that. This entry is for obvious purposes of catharsis, emotional discharge or something in a format that is not criticized because it is not my work, but the work of mental forces that I do not control. So do not expect quality, and probably not feeling either because I do not have it very refined. In this case the date of flight is well planned; I would like to move it forward a couple of weeks but we do what we can. Anyway, I stopped preparing all the finals except one to be able to leave early and without pain or glory, so I'm not at all satisfied with my academic performance in this last stage of the year. But this stage is a stage where nothing is fought anymore; when everything has been promoted or regularized or even free is a matter of inertia, procastination, or some mysterious force of those. In such a way that my vacations have begun, for not even mentioning Mina Clavero, prematurely. And they will start prematurely as soon as I return to Corrientes to walk those corners that I am so eager to travel, or to go out for those posthumous night bike rides that I usually give a day or two before returning to this metropolis from the shell of his mother, to put it mildly. I am not far away, and the date is not far away, but in any case if I do not leave I feel locked up. I feel cold beer running through my throat in a public place, finally!, in a public place that the people of Cordoba consider utopian, because there is no famous Code of Fouls. And on top of that, if that was not enough, there is beach and field. All those things that seemed the worst of the imbalances, being so unbalanced now seem peace, the peace that escaped. I would like to look for the etymology of the word perfect; but it goes back to something that, by its own definition, can no longer be improved. Tell me if those perfect paths can be improved; they can not. Retrotraigo to this beautiful fragment of a traveler with a lot of experience to the darkness and the acrid stench of dust that is in this blog that nobody visits. I do not want to stop with epigraphs; the image is enough. We were standing very close to a bend, so you can see that he saw us as soon as he turned, because he stopped in the act and got a few meters away. While we were in the car - with the radio at maximum, so I heard half of the things - he told us that he had been a backpacker for 30 years and that he liked to travel a lot. He was married to a German and had traveled to Europe about 8 times. We tell you about travel, bubbles and all those cute little things. Or at least they are people who have sympathy. How to dream is free, or at least very cheap, I am dreaming of a moment in which I make another trip to finger with the Alcero parcero and with anyone who wants to adhere to the cause. The experience of this Saturday may be a little different from this one, but not enough so that it does not bring back memories. I am seeing the path that I have to travel in theory; making all the schemes that mentally served me and a lot in those times of constant wandering. The wildest controversies are those that deal with issues for which there is no good evidence from either party. Persecution is common in theology, not in arithmetic, because in arithmetic there is knowledge, while in theology there is only opinion. I will try to be more figurative, because that is what I rescue from the best philosophers: those of clarity. Basically I just wrote a micro-book. After correcting it completely, it was exactly one hundred words. I still introduced a couple of more corrections, always maintaining this balance. Alonso told me about a contest in Spain where you can send stories in up to four or five languages, whose maximum word limit is one hundred; exactly the words that I got. I can not deny that I am proud of this creation although I do not think about sending it to this contest, but more grateful I am with it because it makes me reflect on some things. In simple words, "Letters do not form writers". More vehemently: "you will never be a writer if you are a graduate of letters". And now, after having fought a long time, I see clearly why. The circumstance of creation just showed me the origin of the process. It consists of such a clear connection with an art form that the diffuse essence comes first and then the words come to express it. All this experience of creation was, in my case, fortuitous. I know of people who live materially from these experiences, perhaps because they know how to induce them or know how to take better advantage of them. The experiences of creation have to do, although in a mysterious way, with the emotion, the feeling and the mastery of a technique that is intuited more than what is studied. The field of philosophy, reasoning, logic and the mental organization of experiences is a field connected to the first, but by ties that have nothing to do with creation itself. It has to do with what Sofi told me. The most valuable tool for those who exercise and want to perform in this field is memory: the most valuable method, in my opinion, is the one that allows you to relate information to draw conclusions. The conclusions will have as consequence, on an emotional level, an apprehension before the multiplicity of the real. This observer, critical but cold, can not be an artist at the same time. The artist knows how to abstract to an emotion. The observer knows how to abstract from emotions. The muse's song, in my opinion, is distant to him; What happened to me just does not happen more than fortuitously. I am satisfied with the result because it is a completely new territory. But I could never learn, being as I am now, how to induce the song of the muse. Everyone says that inspiration is something that comes randomly and surprises us anywhere. I do not put this in discussion because of inspiration I know nothing, but here is where the thing becomes immediately suspicious. I see it today more true than ever: the writer could never study Letters without losing something of his essence, as he became more a dynamic network of knowledge than a soul dedicated to perfecting e-motive and inspiring his technique. I hate to think that artists have eminence over critics, but everything ends in this; one experiences the experience, the other discusses it. I chose my career and chose my specialization and chose which path to take from these two, although I am just beginning to glimpse its consequences. None of this is permanent or definitive; I do not regret being the subordinate to special beings who develop their mind and soul in mysterious ways. It is my vocation and I see it clearly. Because of all this, I will always be a slave to my words, the same words that I wrote long ago or recently, and they seem stupid to me. And I will be both a slave to the words of others, which is perhaps more serious. I want to offer surprises to my readers, who after these breaks will be very few; I hope to get your attention after the hiatus, once it happens. It's already decided, and I'm still determined until the end of the month, with 20 days left before the hiatus I'm talking about ends. So far is the most productive month and interesting; paradoxically to the closure. What is undeniable is a freedom in the format of writing that I did not have long ago, but I could not say that it was limited by the influx of visits. Anyway I am enjoying writing these entries, change the format every time and even put a title in Chinese. I got distracted by singing something else and I do not know what I was writing; It does not matter and I do not want to reread to pick up the thread of the conversation. The times change, they say, so perceptibly that one would think that it is totally artificial. This system removes freedoms, like all systems, but makes things go forward and have a course to be productive. The other day I thought if all the people thought the same way I did: from the general to the particular. Without the map, I do not walk the territory. Walking the territory to draw the map, when others already did, seems superfluous to me. Lucio: -I'll do a quick consultation via twitter. I'm trying to understand what a rhizome is. Artemis: -It is a semi-subterranean structure, which opposes the tree structure. Wanting to get close to many things I moved away from conceptions such as: being wise or being creative or being an artist, of the most tormented. So, none of you who will come to my blog in a little less than a month, wait here to find something you definitely hope for. The title of the blog is in Chinese. I had a slight approach to Chinese culture, especially music and some painters; it does not compare in anything with knowing authentically something of the Chinese culture. I took some elements of a couple of products, of the newest in reality, to appreciate them according to my western mentality that will never fully understand them. Such a feat would be great, but just for being great, it makes me titanic and I do not think I have time to fulfill it, given that I also have many things planned. The Chinese alphabet is, it is known, much more difficult; one is adrift copying and pasting incomprehensible symbols that seem to be names. In this sense knowing how to read would be excellent, and knowing how to interpret them in all their millennial meaning would be even better; and this is when I see that the task becomes absurd. Anyway I was able to rescue a couple of records that represent the best thing that happened to Chinese instrumental music in contemporary times. Do not make me go through anything; I'm nothing. I am a young student with an Internet connection. And interests of the most volatile. The lighting of your face maxikiosco lights by; speed up the pace on the pedestrian path, but in reality without caring much. The thing happens for not having a balcony where to plant a ficus fucking and for the stones of the cat nailed to the feet, every time you are encouraged by those works of forced spirituality, to walk barefoot. The thing is to live this same thing every day ad absurdum and in a moment to get bored and then say "I'm going to take action". The methods are many and well debatable. Suddenly you see yourself in a greenish mountain. Everything is so different a black trunk or a maxikiosco 24. It is part of the same sun, but the problem is where we are, that we are ignoring it. After all, one would always have to enjoy where it is. And if it is not possible, there is something that is malfunctioning in us or in the others. The phoenixes play on their terrace. The phoenixes left, the river still flows, alone. Flowers and grass cover the dark road where Go's dynastic house stood. Shin's bright clothes and shining coatings are now the basis of old hills. Now the high clouds cover the sun and I can not see the distant Choan and I am sad. This lila is defoliated, from itself falls and hides its ancient shadow. I'm not passionate about Alejandra, but I was reading a collection of her poems framed within a collection of books about madness. This one seemed great to me; the others did not seem like a great thing to me. I do not want to complain about everything and that's why this epigraph goes in small letters. This poet will surprise me someday, but not today. Succeeding in a foreign city and returning to the city where we come from, is bringing with us a symbolic capital that is victory. This I read in a magazine article, I can not remember which, five minutes have passed. I think that in adopting its rhythm and understanding its customs, understand its turns. After all, there are only experiences. They have said that blogs are a blog of these experiences, that among other things that make up life. "It works," says a Turkish proverb, "It's about fulfilling the role that we play, it's nothing symbolic." On large scales of time you can see that in truth there never was anything really new. When you say that you want to make art as pessoas falam: "Nossa, arrogant! Na verdade, é isso mesmo, sabe? Nao devia be feio falar isso and want isso. Fazer arte é fazer a coisa com afeto e nao deixar directing pela razao da conseqüência. Nao fazer a coisa comecando pela conseqüência, that é o resulta. Fazer, e a conseqüência vai ser o que for. It is estimated that there will be no criticism, tao assim. It's embarrassing to say, more in the end. Actually, that's the same, you know? It should not be ugly to say that or want that. To make art is to do the thing with affection and not allow oneself to be directed by the reason of the consequence. Do not do the thing starting with the consequence, which is the result. Do, and the consequence is going to be whatever it is. If everything was fine, it would not be such a criticism. In traditional philosophy, the literary work is considered as a rhetorical envelope in which the hidden wisdom of the Idea sleeps, to which the reader must awaken with the semiological kiss. Deconstruction will affirm that the rhetorical envelope is all there is and that is why the literary work of art is irreducible to an idea or a concept. Tomás spoke today of having his own garden of figs and a part-time job as a teacher, once he has finished his time in the race. Devendra Banhart dreamed of reincarnating in a seahorse, once all his expectations had been exhausted as an idiotic accumulation of material goods. An imagined, metaphysical solution, probably not feasible; but we do not know if it is also the fig orchard. Hesse speaks of a modest life cloistered in the pursuit of an inalienable personal good: his own vocation. The sage pursues interests that are the fine expression of the wisdom of the world but that at the same time do not interest anyone. In the period of partials something very simple happens with the mind of a student who absorbs everything like a sponge: in the absence of precise terminology the metaphors go towards the desert. Especially in Córdoba capital, which is quite blotting. I checked today as I was walking back home, after performing one of the many tests of these hot days, and actually having to pay another one that I postponed for convenience. There comes a time when the mind becomes saturated with signs: it is tiring to have to translate signs all the time; signs everywhere. I count this poorly with the kettle set for cooked mate, which is known to have anesthetic effects. I say this, to be able to sleep because it's almost five thirty in the morning, and I have a short day ahead, but it must be started early. As a kid I liked to travel with my mind. My favorite destinations at that time were those that dealt with science fiction; my family used to give me books of this style, mostly very naive and that marked me forever. Hitting a big jump on my timeline, I remember having a predilection for those land destinations but still impossible as New York. I also dreamed of gypsy caravans or freighthopping in the heart of North America. Many things induced by the cultural products that I decided to consume in this or that period of my life. Today I am in that moment of inquiry about things that ceased to exist a long time ago: just remember my none-too-urgent obligation to study the Greek tragedy for Wednesday or Thursday. I want to tell briefly how I came to this obsession that occupied me all night, so that now, at half past five, I would like to continue developing but I need a cocido mate to placate and put me to sleep. I'm downloading it to see it whole. In the first ten seconds of the program a lute music played in the background: I wanted to know more about this music, specifically names of artists or names of compositions. Medieval music remains a mystery, and is composed mostly of choral songs in praise of the Christian god, which were composed by great virtuosos mostly Italian or Iberian. But these choral songs do not interest me in the least; Inquiring about instrumental music, I came to the conclusion that they all had an instrument in common: the lute. I'm not going to describe technically what a lute is, but how it sounds: as we all imagine the Middle Ages. As a result of this inquiry came the present album, which I present to you: an album with fifteen songs that have in common a lute and a passionate composition. I could not help but fall into it, enchanted, and already started in names surely proceed to a very long obsession. Everything begins and ends, for today, in this album of fifteen songs of which I can boast as an articulation totally mine. Click on Juana la Loca for your delight.
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